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Q: What is the minimum quantity of sample required for counting?
A: The disposable counting chamber contains 18-20 microliters of sample solution.

Q: Do I need any special buffer or reagent for cell counting?
A: No. Special buffers are not required. Cells that are suspended in growth media, PBS, or other buffers are routinely counted. Trypan blue is used to stain dead cells.

Q: What is the cell size range for Cellometer Auto T4?
A: Cellometer Auto T4 is suitable for cell diameters ranging from 5 to 40 micron.

Q: What is the cell size range for Cellometer Auto M10?
A: Cellometer Auto T4 is suitable for cell diameters ranging from 2 to 12 micron.

Q: What are the limits of cell concentration (minimum and maximum) for Cellometer Auto T4?
A: The limits of cell concentration depend on the cell size to a certain degree. Large cells have a lower minimum limit and small cells have a higher maximum limit. The common cell concentration range for reliable counting is from 0.25 x 10^6 to 1 x 10^7 cells/ml. For medium to large cells, the minimum concentration can go down to 0.1 x 10^6 cells/ml. For small cells, the maximum can go up to 15 x 10^6 cells/ml.

Q: What is the principle of operation for Cellometer® Automatic Cell Counter?
A: The basic principle of operation is imaging cytometry. It uses a disposable cell counting chamber to spread cells into a thin liquid layer, as is the case for traditional hemacytomer. The total counting area for Auto T4 is equivalent to all four corner squares of hemacytometer. Cellometer® automates cell counting by taking images of cells in the counting chamber, analyzing the number of cells in each image using image analysis, and converting the number of cells into cell concentration.

Q: Does Cellometer® measure viability?
A: Yes, when trypan blue is added to the sample, live cell concentration and viability are both measured.

Q: Does Cellometer Auto T4 count trypan blue positive and negative cells at the same time in the same sample?
A: Yes. If trypan blue stained cells are loaded into the counting chamber and “Test Viability” option is selected, both trypan blue positive and negative cells are measured at the same time, from the same sample.

Q: Can Cellometer Auto T4 count my cells?
A: Cellometer Auto T4 systems are being used for cell counting in a broad range of laboratories, which study a large number of cell types. See Cell Image Gallery on this website for details. If your cells are not listed, please contact Nexcelom Bioscience directly.

Q: Can Cellometer Auto T4 count cells based on size?
A: Yes, Cellometer Auto T4 is an image-based cell counter. The user can set minimum and maximum size range for counting.

Q: Will cell debris be counted?
A: No. In a typical cell culture, cell debris is smaller in size and different in morphology than live cells. Cellometer® counting software excludes debris.

Q: What happens to the clumpy cells?
A: Small cell clumps are separated using special software.

Q: Can I count irregular shaped cells?
A: Yes.

Q: How fast can I count one sample?
A: Counting time depends on the number of cells, morphology of the sample, as well as the computer. Typically, cells lines require 15 to 30 seconds per sample.

Q: Does Cellometer Auto T4 need routine maintenance?
A: No. There is no liquid handling system inside the Auto T4. The disposable counting chamber contains the sample and is removed when cell counting is done. Consequently there is no need for cleaning or routine maintenance.

Q: Do I need a computer to operate Cellometer Auto T4?
A: Yes. Cellometer Auto T4 is connected to a PC via USB cable. An existing PC in your lab running another instrument may be used. You may also purchase an optional laptop computer, with Cellometer software loaded, as part of your system.

Q: What are the computer requirements?
A: Computer Recommendations: Windows XP 2.4 GHz or higher speed processor 512 MB Memory USB 2.0 port (required). Cellometer can also be run on an Intel based Mac running Parallels/WinXP. Click here to learn more.

Q: Our lab uses a Mac, can I run Cellometer on a Mac?
A: Yes. Using an Intel-based Mac with Parallels Desktop 3.0 (with Windows XP), Cellometer is easily run on a Mac. Click here to learn more.

Q: Can Cellometer® Cell Counter count Yeast cells?
A: Cellometer model Auto M10 is designed for counting yeast, algae, and platelets

Q Does Cellometer® use a Standard sized slide?
A: CHT4 is an all-plastic disposable hemacytometer produced using high quality optical materials. It is specially designed to fit Cellometer® cell counters.

Q: Can a sample with low cell viability be counted?
A: Yes, samples with viability as low as 14% have been counted.

Q: Does Cellometer® Cell Counter count primary cells or cell lines?
A: Most mammalian cells can be counted, including both primary cells and cell lines. More than 150 types of cells are counted using Cellometer® Cell Counters, Cell Library shows cell images and counting results.

Q: Can Cellometer® Cell Counter count bacteria?
A: Cellometer model Auto T4 and model Auto M10 cannot be used to count bacteria. If you have specific requirements, please contact info@nexcelom.com

Q: Can Cellometer® Cell Counter count platelets?
A: Yes, model M10 is suitable for counting platelets.

Q: What is the operational cost?
A: The only operation cost of Cellometer® Cell Counter is the disposable counting chamber. There is no special reagent required for the instruments. Since there is no liquid handling system within the cell counter, liquid clogging is eliminated.

Q: Can Cellometer® Cell Counter measure cell size?
A: Yes, for each cell counting operation, cell size histogram is automatically generated. Cell size data can be saved.

Q: How many samples can I plot on the same cell size histogram?
A: If you save cell size data, up to five samples can be plotted on the same cell size histogram.